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植物種苗團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構生物技術課Establishment of the Acc

「植物種苗團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構生物技術課Establishment of the Acc」於資料集「種苗研發成果」由單位「農業部」的陳乃華小姐所提供，聯繫電話是04-2582-5425，最近更新時間為：2023-07-13 01:04:11。欄位年度的內容是2012 ，欄位計畫名稱_中文的內容是植物種苗團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構 ，欄位計畫名稱_英文的內容是Establishment of the Accreditation system for plant seedlings ，欄位計畫編號_中文的內容是101農科-9.2.3-種-X2 ，欄位計畫編號_英文的內容是101AS -9.2.3-SS-X2 ，欄位主辦單位_中文的內容是生物技術課 ，欄位主辦單位_英文的內容是Biotechnology Section ，欄位主辦人_中文的內容是莊淑貞 ，欄位主辦人_英文的內容是Su-Jean Chuang ，欄位中文摘要的內容是1.種苗檢測技術認驗證TAF實驗室設備建構及維護：本年度實驗室之儀器建置與驗證。新購置儀器(包括核酸定量儀、聚合酵素梯度溫度連鎖反應儀、植物生長箱、微量分注器以及桌上型離心機)符合TAF檢測實驗室需求之確認及各廠牌測試比較，並已完成採購及驗收程序。完成實驗室已設置儀器(包括ELISA讀值儀、微量分注器、酸鹼度計及聚合酵素溫度連鎖反應儀等)之年度校正維護，以符合TAF檢測實驗室管理。 2.瓜類退綠黃化病毒及草莓潛隱輪斑病毒驗證體系建構：參酌國內外文獻，已完成瓜類退綠黃化病毒（Cucurbit chlorotic yellows virus,CCYV）與草莓潛隱輪斑病毒（Strawberry latent ringspot virus, SLRSV）之檢測技術盤點。瓜類退綠黃化病毒目前檢測技術主要有：RT-PCR、Western blotting、ELISA及Immunoelectron microscopy；草莓潛隱輪斑病毒目前檢測技術主要有：RTPCR、IC-RT-PCR、ELISA與病徵判別。以不同的樣品進行各種檢測方法的方法測試，挑選最適合的檢測方法做為標準檢測流程。 3.種子(苗)品質純度分子檢測技術研發及標準化：種子公司番茄品種植體材料(雜交一代及父、母本親)，及種苗場番椒新品種種苗2號及4號，共3組9個材料。番椒共篩選出8/100組有用引子，11個識別片段。番茄共篩選出7/100組有用引子，7個識別片段。經回收、定序、解序及重新設計條帶專一性引子，番茄SCAR標誌7組增幅出6個專一性條帶，番椒SCAR標誌10組增幅出6個專一性條帶。以番茄採種種子為材料，及以5個番茄識別標誌，進行F1種子純度分子檢測。檢測結果，顯示2個雜交種子其花粉為來自外來花粉非雜交種之父本親，本批番茄採種種子的F1種子純度為97.6%。 4.十字花科種子健康檢查驗證體系之建立：本計畫目的係為準備種子檢查室十字花科種子黑腐病菌檢測認證工作，已依國際種子檢查協會規定之檢測方式建立適合本實驗室之十字花科種子黑腐病菌檢測流程。實際以人工汙染種子進行檢測流程的測試，可於FS及mCS20ABN兩種選擇性培養基上觀察到測試菌落的標準型態；在病原性測試也可將分離出之黑腐病菌菌株接種於甘藍葉片上顯示出典型病徵；靈敏度測試結果亦可測得達0.02%的帶菌率。，欄位英文摘要的內容是The purpose of this plan first is to promote the plant pathogens detection and isolation efficiency of the laboratory. This year,regarding TAF-certification for laboratory equipment, design and layout of the laboratory has already been completed and laboratory bench and instruments have been acquired, calibrated and approved for operations.Second purpose of this plan, with reference to published literature,detection methods for Cucurbit chlorotic yellows virus, CCYV and Strawberry latent ringspot virus, SLRSV have been researched and cataloged. Current detection methods regarding Cucurbit chlorotic yellows virus, CCYV include: RT-PCR, Western blotting, ELISA, and Immunoelectron microscopy; for Strawberry latent ringspot virus, SLRSV, current detection methods include: RT-PCR, IC-RT-PCR, ELISA as well as symptom diagnosis. Utilizing various detecting methods on different samples, the most suitable detection method will be chosen as the standard detection procedure. One required index of high quality of seed/seedling is genetic purity, especially for hybrid seed. Genetic purity in a commercial hybrid of solanaceous crops is very important. This year, we setup this quality control mode by using molecular markers in seed production system of the crops of tomato and pepper, last year we did some part work in tomato. We obtain seven sets of SCAR primer for six specific DNA fragments in tomato and ten sets of SCAR primer for six specific DNA fragments in pepper,they can identify hybrid from parents. The SCAR primer can have the sharp and easy-read DNA fragment after PCR amplification and electrophoresis.We will collect these SCAR primers as members of data base for testing F1 hybrid seed purity in tomato and pepper till year 2013. Last object of this program is for the ISTA accreditation on the seed testing of Xanthomonas campestris pv. campestris (Xcc) which causes black rot of crucifers. The Xcc testing procedure which conforms to ISTA rules has been established. Artificial infested seeds were adopted to check the Xcc testing procedure. On FS and mCS20ABN selective media, Xcc showed individully pale green and pale yellow mucoid colonies. The cabbage leaves that were stab-inoculated with Xcc showed typical yellowing necrotic V-shaped symptoms in pathogenicity test. The Xcc-carried ratio of 0.02% could be detected in sensitivity test. 。

年度

2012

計畫名稱_中文

植物種苗團隊-植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構

計畫名稱_英文

Establishment of the Accreditation system for plant seedlings

計畫編號_中文

101農科-9.2.3-種-X2

計畫編號_英文

101AS -9.2.3-SS-X2

主辦單位_中文

生物技術課

主辦單位_英文

Biotechnology Section

主辦人_中文

莊淑貞

主辦人_英文

Su-Jean Chuang

中文摘要

1.種苗檢測技術認驗證TAF實驗室設備建構及維護：本年度實驗室之儀器建置與驗證。新購置儀器(包括核酸定量儀、聚合酵素梯度溫度連鎖反應儀、植物生長箱、微量分注器以及桌上型離心機)符合TAF檢測實驗室需求之確認及各廠牌測試比較，並已完成採購及驗收程序。完成實驗室已設置儀器(包括ELISA讀值儀、微量分注器、酸鹼度計及聚合酵素溫度連鎖反應儀等)之年度校正維護，以符合TAF檢測實驗室管理。 2.瓜類退綠黃化病毒及草莓潛隱輪斑病毒驗證體系建構：參酌國內外文獻，已完成瓜類退綠黃化病毒（Cucurbit chlorotic yellows virus,CCYV）與草莓潛隱輪斑病毒（Strawberry latent ringspot virus, SLRSV）之檢測技術盤點。瓜類退綠黃化病毒目前檢測技術主要有：RT-PCR、Western blotting、ELISA及Immunoelectron microscopy；草莓潛隱輪斑病毒目前檢測技術主要有：RTPCR、IC-RT-PCR、ELISA與病徵判別。以不同的樣品進行各種檢測方法的方法測試，挑選最適合的檢測方法做為標準檢測流程。 3.種子(苗)品質純度分子檢測技術研發及標準化：種子公司番茄品種植體材料(雜交一代及父、母本親)，及種苗場番椒新品種種苗2號及4號，共3組9個材料。番椒共篩選出8/100組有用引子，11個識別片段。番茄共篩選出7/100組有用引子，7個識別片段。經回收、定序、解序及重新設計條帶專一性引子，番茄SCAR標誌7組增幅出6個專一性條帶，番椒SCAR標誌10組增幅出6個專一性條帶。以番茄採種種子為材料，及以5個番茄識別標誌，進行F1種子純度分子檢測。檢測結果，顯示2個雜交種子其花粉為來自外來花粉非雜交種之父本親，本批番茄採種種子的F1種子純度為97.6%。 4.十字花科種子健康檢查驗證體系之建立：本計畫目的係為準備種子檢查室十字花科種子黑腐病菌檢測認證工作，已依國際種子檢查協會規定之檢測方式建立適合本實驗室之十字花科種子黑腐病菌檢測流程。實際以人工汙染種子進行檢測流程的測試，可於FS及mCS20ABN兩種選擇性培養基上觀察到測試菌落的標準型態；在病原性測試也可將分離出之黑腐病菌菌株接種於甘藍葉片上顯示出典型病徵；靈敏度測試結果亦可測得達0.02%的帶菌率。

英文摘要

The purpose of this plan first is to promote the plant pathogens detection and isolation efficiency of the laboratory. This year,regarding TAF-certification for laboratory equipment, design and layout of the laboratory has already been completed and laboratory bench and instruments have been acquired, calibrated and approved for operations.Second purpose of this plan, with reference to published literature,detection methods for Cucurbit chlorotic yellows virus, CCYV and Strawberry latent ringspot virus, SLRSV have been researched and cataloged. Current detection methods regarding Cucurbit chlorotic yellows virus, CCYV include: RT-PCR, Western blotting, ELISA, and Immunoelectron microscopy; for Strawberry latent ringspot virus, SLRSV, current detection methods include: RT-PCR, IC-RT-PCR, ELISA as well as symptom diagnosis. Utilizing various detecting methods on different samples, the most suitable detection method will be chosen as the standard detection procedure. One required index of high quality of seed/seedling is genetic purity, especially for hybrid seed. Genetic purity in a commercial hybrid of solanaceous crops is very important. This year, we setup this quality control mode by using molecular markers in seed production system of the crops of tomato and pepper, last year we did some part work in tomato. We obtain seven sets of SCAR primer for six specific DNA fragments in tomato and ten sets of SCAR primer for six specific DNA fragments in pepper,they can identify hybrid from parents. The SCAR primer can have the sharp and easy-read DNA fragment after PCR amplification and electrophoresis.We will collect these SCAR primers as members of data base for testing F1 hybrid seed purity in tomato and pepper till year 2013. Last object of this program is for the ISTA accreditation on the seed testing of Xanthomonas campestris pv. campestris (Xcc) which causes black rot of crucifers. The Xcc testing procedure which conforms to ISTA rules has been established. Artificial infested seeds were adopted to check the Xcc testing procedure. On FS and mCS20ABN selective media, Xcc showed individully pale green and pale yellow mucoid colonies. The cabbage leaves that were stab-inoculated with Xcc showed typical yellowing necrotic V-shaped symptoms in pathogenicity test. The Xcc-carried ratio of 0.02% could be detected in sensitivity test.