""2014"The main purpose of this project “Quantum Dots-BioChip De
「""2014"The main purpose of this project “Quantum Dots-BioChip De」於資料集「EPQSummary_EnvironmentalMonitoringAndTesting」由單位「行政院環境保護署」的陳先生所提供，聯繫電話是02-2311-7722#2386，最近更新時間為：2023-07-30 01:01:45。 欄位Project Title的內容是"" ， 欄位Project Subject的內容是"The main purpose of this project “Quantum Dots-BioChip Detection System for Environmental Microbes (2/4) -Application of Gene Chip in Endocrine Disrupter Substance Assay” is to combine two technologies included yeast screening and aptamer screening. The double expression dual plasmid yeast system can be applied for primary screening and the aptamer test can detect the specific Endocrine Disrupter Substance. Five different endocrine disrupter substance including bisphenol-A、p’p’-DDE、Estrone、17β estradiol and genistein were tested in our yeast system. The results of detection limit, EC and TEQ are similar from fluorescence assay and β-Galactosidase activity assay of our yeast system. For example, detection limit of 17β estradiol in fluorescence test is 5x10-11 (g/L) with R2=0.98 among 10-9~10-11 (g/L) and EC50 is 2.1x10-10 (g/L). For β-Galactosidase activity of 17β estradiol in this system, the detection limit of our system is 5x10-12 (g/L) with R2=0.97 among 10-6~10-12 (g/L) and EC50 is 1.4x10-9 among 10-9~10-11 (g/L). Our system is 100 times of sensitivity than current yeast system. The aptamer will be applied with previous technology that was developed by previous EPA project (Gene-Quantum Dots Detection System for environmental pathogen, (EPA-101-U1U4-04-001)). This novel technology utilized microbead linked with specific oligonucleotide and detected by quantum dots. To develop the aptamer system, we used 3D softare to simulate aptamer structures that are specific to Bisphenol A and 17β estradiol. We have designed one BPA-aptamer and two 17β estradiol-aptamer structures and tested by bisphenol-A、p’p’-DDE、estrone、17β estradiol and genistein. 17β estradiol of aptamer-microbead system have 100% specificity below 100nM. Both aptamer- microbead systems have 100% sensitivity with 1nM of detection limit. However, the quality and quantity detection ability of both aptamer- microbead systems required future research to improve" ， 欄位Project Year的內容是2014 ， 欄位Organizer的內容是"Environmental Analysis Laboratory" ， 欄位Executive Unit的內容是"中原大學" ， 欄位Reporting download URL的內容是https://epq.epa.gov.tw ， 欄位Publish Date的內容是20150201 。
Project Title
""
Project Subject
"The main purpose of this project “Quantum Dots-BioChip Detection System for Environmental Microbes (2/4) -Application of Gene Chip in Endocrine Disrupter Substance Assay” is to combine two technologies included yeast screening and aptamer screening. The double expression dual plasmid yeast system can be applied for primary screening and the aptamer test can detect the specific Endocrine Disrupter Substance. Five different endocrine disrupter substance including bisphenol-A、p’p’-DDE、Estrone、17β estradiol and genistein were tested in our yeast system. The results of detection limit, EC and TEQ are similar from fluorescence assay and β-Galactosidase activity assay of our yeast system. For example, detection limit of 17β estradiol in fluorescence test is 5x10-11 (g/L) with R2=0.98 among 10-9~10-11 (g/L) and EC50 is 2.1x10-10 (g/L). For β-Galactosidase activity of 17β estradiol in this system, the detection limit of our system is 5x10-12 (g/L) with R2=0.97 among 10-6~10-12 (g/L) and EC50 is 1.4x10-9 among 10-9~10-11 (g/L). Our system is 100 times of sensitivity than current yeast system. The aptamer will be applied with previous technology that was developed by previous EPA project (Gene-Quantum Dots Detection System for environmental pathogen, (EPA-101-U1U4-04-001)). This novel technology utilized microbead linked with specific oligonucleotide and detected by quantum dots. To develop the aptamer system, we used 3D softare to simulate aptamer structures that are specific to Bisphenol A and 17β estradiol. We have designed one BPA-aptamer and two 17β estradiol-aptamer structures and tested by bisphenol-A、p’p’-DDE、estrone、17β estradiol and genistein. 17β estradiol of aptamer-microbead system have 100% specificity below 100nM. Both aptamer- microbead systems have 100% sensitivity with 1nM of detection limit. However, the quality and quantity detection ability of both aptamer- microbead systems required future research to improve"
Project Year
2014
Organizer
"Environmental Analysis Laboratory"
Executive Unit
"中原大學"
Reporting download URL
Publish Date
20150201
「""2014"The main purpose of this project “Quantum Dots-BioChip De」所屬的資料集：「EPQSummary_EnvironmentalMonitoringAndTesting」的其他資料
Project Title：
"Environmental Ambient Water Quality Monitoring of Middle Taiwan"
Project Subject：
"The aim of this project was to properly understand the changing trend of water quality in those typ
Project Year：
2013
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Probing the correlation between the organic contamination level in soils and the actual contaminati
Project Subject：
"Concentrations of organic contaminants in common productive soils based on the total soil mass give
Project Year：
2015
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Monitoring Project for the River ＆ Ocean Water Quality of Taichung City in 2014"
Project Subject：
"To achieve the goal of cleaning river water, several pollution control actions had been enforced by
Project Year：
2014
Organizer：
"Environmental Protection Bureau, Taichung City"
Project Title：
"Project of Background Investigation for Chemical Substances Environmental Distribution, 2018"
Project Subject：
"The major aims of this project were to (1) investigate concerned chemicals in fifteen Taiwanese riv
Project Year：
2018
Organizer：
"Toxic and Chemical Substances Bureau"
Project Title：
"Microextraction technology Development (2/4) – Extraction Method Development for Pesticides in Wate
Project Subject：
"This project is to investigate the determination of 131 pesticides in water by microextraction tech
Project Year：
2014
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"The Environment Quality Monitoring of Taoyuan County (2010)"
Project Subject：
" This project focuses on monitoring the noise levels, river water and ground water qualities of Ta
Project Year：
2010
Organizer：
"Taoyuan County Government Environment Protection Bureau"
Project Title：
"Fine Particulate Matter (PM2.5) Chemical Speciation Monitoring Project during 2015-2016"
Project Subject：
"This project collected PM2.5 for chemical speciation at the air quality monitoring stations of Taiw
Project Year：
2015
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Smart Environmental Application Development Plan"
Project Subject：
"In response to the requirements of sensing application analysis and the integrated display of the a
Project Year：
2018
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Developing Environmental Analytical Methods for Nanomaterials (2/4)- Analytical Methods of Fulleren
Project Subject：
"Large amounts nanomaterials are fabricated as versatile products in the markets with the fast devel
Project Year：
2014
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Fine Particulate Matter (PM2.5) Chemical Speciation Monitoring and Analysis Project"
Project Subject：
"This study conducted an assigned mission of PM2.5 chemical speciation monitoring at Douliu, Chiayi,
Project Year：
2016
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Management and Auditing of Environmental Water Quality Monitoring Program(2018.04~2021.09)"
Project Subject：
"The major goal of this project was to check the whole processes that influenced the monitoring data
Project Year：
2018
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"The Environment Quality Monitoring of Taoyuan County(2014)"
Project Subject：
"In this report ”The Environmental Quality Monitoring in Taoyuan County (2014)”, we choose several k
Project Year：
2014
Organizer：
"Taoyuan County Government Environment Protection Bureau"
Project Title：
"The Operations Management of Environmental Protection Bureau of Yunlin County analysis laboratory"
Project Subject：
"The Operations Management of Environmental Protection Bureau of Yunlin County analysis laboratory l
Project Year：
2017
Organizer：
"Environmental Protection Bureau Yunlin County"
Project Title：
"Analytical Technology Study for Environmental Nanoparticle and Green Chemistry (3/3) - Development
Project Subject：
" The plan is to develop the nanoparticles separation technology for biotoxicity testing in envir
Project Year：
2017
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"2016 Environmental Quality Monitoring Station(including Mobile Monitoring Vehicle) Operation And Ma
Project Subject：
"This project was implemented from Jan. 12, 2016 to Dec. 31, 2016. All tasks defined in the Environm
Project Year：
2016
Organizer：
"Environmental Protection Bureau, Taichung City"
Project Title：
"Environmental radio frequency non-ionizing radiation information investigation and monitoring"
Project Subject：
"This project aimed to understand the difference of electromagnetic exposure before and after the in
Project Year：
2021
Organizer：
"Department of Air Quality Protection ＆amp; Noise Control"
Project Title：
"106 Annual Report on the Achievements of Hualien County Air Quality Cleaning Area and Air Quality M
Project Subject：
"(A) indoor air quality operations
The inspection work for the second batch of premises has been car
Project Year：
2017
Organizer：
"Environmental Protection Bureau, Hualien County"
Project Title：
"UV-Index Forecasting System and Instrument Calibration Laboratory "
Project Subject：
"The main task of this project, “UV Forecast System and Monitoring Instrument Calibration Lab.-Fisca
Project Year：
2014
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Analyze and investigation of velocity and volumetric flow rate of gas stream in a duct"
Project Subject：
"The volumetric flow rate of complex flow field in duct was experimentally investigated in this proj
Project Year：
2015
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Fine Particulate Matter (PM2.5) Chemical Speciation Monitoring and Analysis Project"
Project Subject：
"This study conducted an assigned mission of PM2.5 chemical speciation monitoring at Douliu, Chiayi,
Project Year：
2016
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"The study of surface-enhanced Raman scattering spectroscopy in environmental Forensics"
Project Subject：
"The goal of this project is the development of a high-speed analytic platform for toxicants in wate
Project Year：
2015
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Project of environmental sampling and analysis for health risk assessment at Dalinpu area in Kaohsi
Project Subject：
"This project started from October 3rd 2017 and was end on November 30th 2018. The goal of the proje
Project Year：
2017
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Tracing sources of metal pollutions in the aquatic environment using MC-ICP-MS (1/2)"
Project Subject：
"The aim of this study is to trace possible sources of metal pollutions in the aquatic environment.
Project Year：
2020
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"APMIS and PSN Data Quality Improvement Project"
Project Subject：
"In order to strengthen the application of the information application management system function of
Project Year：
2019
Organizer：
"Environmental Analysis Laboratory"
Project Title：
" Research on RF Non-Ionizing Radiation Regional Environmental Monitoring"
Project Subject：
"Plan and implement the establishment of an electromagnetic wave area monitoring system and measurem
Project Year：
2019
Organizer：
"Department of Air Quality Protection ＆amp; Noise Control"
Project Title：
"Environmental Ambient Water Quality Monitoring of South Taiwan "
Project Subject：
" The main goal of this project is to monitor the water quality of nine cities and counties in the
Project Year：
2013
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
Project Title：
"Development of toxicity tests for evaluating the sensitivity of biota to estuarine sediments (1/2)"
Project Subject：
"Chemical contaminants discharged into estuaries and coastal areas often attach to sediment particle
Project Year：
2016
Organizer：
"Environmental Analysis Laboratory"
Project Title：
""
Project Subject：
"In this project, the market information of environmentally analytical companies in Mainland China w
Project Year：
2015
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Development of analytical method for PCN project (2/2)"
Project Subject：
"The project for developing the method for the analysis of polychlorinated naphthalene (PCNs) in fly
Project Year：
2018
Organizer：
"Environmental Analysis Laboratory"
Project Title：
"Pilot-type pilot project for air quality sensing, Internet of things deployment, maintenance, and d
Project Subject：
"People are more and more aware of the problem of air pollution because that the quality of air will
Project Year：
2017
Organizer：
"Department of Environmental Monitoring ＆amp; Information Management"
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