植物種苗研究團隊-生技種苗檢測服務建置與產業推動生物技術課Industry extension and establishmen
「植物種苗研究團隊-生技種苗檢測服務建置與產業推動生物技術課Industry extension and establishmen」於資料集「種苗研發成果」由單位「農業部」的陳乃華 小姐所提供,聯繫電話是04-2582-5425,最近更新時間為:2023-07-13 01:04:11。 欄位年度的內容是2013 , 欄位計畫名稱_中文的內容是植物種苗研究團隊-生技種苗檢測服務建置與產業推動 , 欄位計畫名稱_英文的內容是Industry extension and establishment of
biotech seedling detection serviceplant seed/seedling team
, 欄位計畫編號_中文的內容是102農科-1.1.2-種-X1 , 欄位計畫編號_英文的內容是102AS -1.1.2-SS-X1 , 欄位主辦單位_中文的內容是生物技術課 , 欄位主辦單位_英文的內容是Biotechnology Section , 欄位主辦人_中文的內容是鍾文全 , 欄位主辦人_英文的內容是Wen-Chuan Chung , 欄位中文摘要的內容是利用multiplex PCR技術進行基因轉殖木瓜檢測,得知Multiplex PCR可取代一般PCR進行基因轉殖木瓜檢測,以達到降低檢測成本,並提升檢測能量之目的。評估SYBR Green、Safe view等2種螢光試劑對基因轉殖大豆與玉米檢測效能,結果顯示SYBR Green分出基因轉殖及非基因轉殖大豆和玉米的能力優於Safe view。訪視6家文心蘭組培場,瞭解其設備規模、生產流程及瓶苗出貨標準,並蒐集市售瓶苗進行調查,探討影響瓶苗品質之外部型態指標,包括葉數、根數、株高、根長、鮮乾重,分析810個樣品發現瓶苗間葉片數並無顯著差異,根數及根長則有較高的變異程度,推測可能是子瓶移植操作的影響,株高仍是較為簡便可靠的分級標準,初步結果建議以區分大中兩級為可出貨品質,小等級植株管理模式還需進一步評估。度拜訪16家蝴蝶蘭組培場,與經營者訪談組培設備、生產流程及瓶苗出貨標準。從11家組培場中蒐集12份蝴蝶蘭V3瓶苗,每份有6瓶子瓶,共計72份樣品。這些瓶苗經合格率調查、莖葉及根的數量與重量調查後,分為4個瓶苗品質等級。取樣品進行植體成份分析,其餘植株上盆栽植,並移到專業的蝴蝶蘭中小苗栽培場栽培到中苗。取中苗再進行合格率、莖葉及根數量與重量調查,並分成4個中苗品質等級,並進行中苗植體分析。優良蝴蠂蘭瓶苗為株型端正、葉型與根正常、三葉以上葉片、二條以上有效根、株重3公克以上。葉的氮素含量中等(乾重1.5-4%),高量的鉀含量(乾重5%以上)、磷含量介於乾重0.4-0.8%之間,鈣含量0.4%以上,鎂的含量在0.15%以上。以逆轉錄聚合酶鏈式反應(revese transcription–PCR,RT-PCR)檢測蘭園蘭花常見病毒齒舌蘭輪斑病毒(Odontoglossum ringspot virus, ORSV)與東亞蘭嵌紋病毒(Cymbidium mosaic virus, CymMV),共檢測1346件瓶苗樣品,檢測結果得知健康種苗比例為92.8%。 , 欄位英文摘要的內容是Multiplex PCR and PCR was apllied to detect GM papaya seedlings.Multiplex PCR had the same result as general PCR, resulting in cost reduction and enhancing detection capacity. We visit six Oncidium tissue culture factories, to understand the company profile, production processes and plantlets standards in this year. Furthermore, we gather commercially Oncidium TC plantlets to explore the influence of the external traits of plantlets quality, including the number of leaves,root number, plant height, root length, fresh/dry weight. Eight hundred and ten young plantlets were found no significant difference between the number of leaves, root number and root length were have a higher degree of variation, presumably by sub-culture transplant operation. Plant height is still relatively easy and reliable grading standards.Preliminary results suggest that large and medium grades are ready for marketing and management model of the small grade plantlets need further evaluation. On the other hand, we also visited 16 Phalaenopsis tissue culture factories and interviewed with the operators about TC equipment,production processes and plantlets standard shipping. We collected 12 portion of Phalaenopsis Sogo Yukidiam ‘V3’ flasks from 11 TC factories,each with 6 flasks, a total of 72 samples. These in vitro plantlets were followed by qualified survey, calculating the numbers and weights of shoots and roots and then divided into four quality levels. We also took some samples to analyze the implant component, and the remaining plants were moved in a professional orchid nursery until they grew to medium size. The medium plants were also measured and analyzed the same item as in vitro plantlets. These medium plants were classified to four grades according to the analysis. Superior Phalaenopsis plantlets have upright type, normal leaf and root shapes, more than three leaves, two or more valid roots and plant weight is higher than 3 grams. These plants also have moderated leaf nitrogen content (1.5-4% dry weight), high levels of potassium (more than 5% dry weight). 04-0.8% phosphorus dry weight content, the calcium content of less than 0.4% and the magnesium content 0.15% or more.1346 samples of Phalaenopsis tissue culture(TC) seedling collected from 3 orchid nurseries were detected by RT-PCR technique for Odontoglossum ringspot virus(ORSV)and Cymbidium mosaic virus(CymMV).Results showed that 92.8% samples were healthy seedlings 。
年度
2013
計畫名稱_中文
植物種苗研究團隊-生技種苗檢測服務建置與產業推動
計畫名稱_英文
Industry extension and establishment of biotech seedling detection serviceplant seed/seedling team
計畫編號_中文
102農科-1.1.2-種-X1
計畫編號_英文
102AS -1.1.2-SS-X1
主辦單位_中文
生物技術課
主辦單位_英文
Biotechnology Section
主辦人_中文
鍾文全
主辦人_英文
Wen-Chuan Chung
中文摘要
利用multiplex PCR技術進行基因轉殖木瓜檢測,得知Multiplex PCR可取代一般PCR進行基因轉殖木瓜檢測,以達到降低檢測成本,並提升檢測能量之目的。評估SYBR Green、Safe view等2種螢光試劑對基因轉殖大豆與玉米檢測效能,結果顯示SYBR Green分出基因轉殖及非基因轉殖大豆和玉米的能力優於Safe view。訪視6家文心蘭組培場,瞭解其設備規模、生產流程及瓶苗出貨標準,並蒐集市售瓶苗進行調查,探討影響瓶苗品質之外部型態指標,包括葉數、根數、株高、根長、鮮乾重,分析810個樣品發現瓶苗間葉片數並無顯著差異,根數及根長則有較高的變異程度,推測可能是子瓶移植操作的影響,株高仍是較為簡便可靠的分級標準,初步結果建議以區分大中兩級為可出貨品質,小等級植株管理模式還需進一步評估。度拜訪16家蝴蝶蘭組培場,與經營者訪談組培設備、生產流程及瓶苗出貨標準。從11家組培場中蒐集12份蝴蝶蘭V3瓶苗,每份有6瓶子瓶,共計72份樣品。這些瓶苗經合格率調查、莖葉及根的數量與重量調查後,分為4個瓶苗品質等級。取樣品進行植體成份分析,其餘植株上盆栽植,並移到專業的蝴蝶蘭中小苗栽培場栽培到中苗。取中苗再進行合格率、莖葉及根數量與重量調查,並分成4個中苗品質等級,並進行中苗植體分析。優良蝴蠂蘭瓶苗為株型端正、葉型與根正常、三葉以上葉片、二條以上有效根、株重3公克以上。葉的氮素含量中等(乾重1.5-4%),高量的鉀含量(乾重5%以上)、磷含量介於乾重0.4-0.8%之間,鈣含量0.4%以上,鎂的含量在0.15%以上。以逆轉錄聚合酶鏈式反應(revese transcription–PCR,RT-PCR)檢測蘭園蘭花常見病毒齒舌蘭輪斑病毒(Odontoglossum ringspot virus, ORSV)與東亞蘭嵌紋病毒(Cymbidium mosaic virus, CymMV),共檢測1346件瓶苗樣品,檢測結果得知健康種苗比例為92.8%。
英文摘要
Multiplex PCR and PCR was apllied to detect GM papaya seedlings.Multiplex PCR had the same result as general PCR, resulting in cost reduction and enhancing detection capacity. We visit six Oncidium tissue culture factories, to understand the company profile, production processes and plantlets standards in this year. Furthermore, we gather commercially Oncidium TC plantlets to explore the influence of the external traits of plantlets quality, including the number of leaves,root number, plant height, root length, fresh/dry weight. Eight hundred and ten young plantlets were found no significant difference between the number of leaves, root number and root length were have a higher degree of variation, presumably by sub-culture transplant operation. Plant height is still relatively easy and reliable grading standards.Preliminary results suggest that large and medium grades are ready for marketing and management model of the small grade plantlets need further evaluation. On the other hand, we also visited 16 Phalaenopsis tissue culture factories and interviewed with the operators about TC equipment,production processes and plantlets standard shipping. We collected 12 portion of Phalaenopsis Sogo Yukidiam ‘V3’ flasks from 11 TC factories,each with 6 flasks, a total of 72 samples. These in vitro plantlets were followed by qualified survey, calculating the numbers and weights of shoots and roots and then divided into four quality levels. We also took some samples to analyze the implant component, and the remaining plants were moved in a professional orchid nursery until they grew to medium size. The medium plants were also measured and analyzed the same item as in vitro plantlets. These medium plants were classified to four grades according to the analysis. Superior Phalaenopsis plantlets have upright type, normal leaf and root shapes, more than three leaves, two or more valid roots and plant weight is higher than 3 grams. These plants also have moderated leaf nitrogen content (1.5-4% dry weight), high levels of potassium (more than 5% dry weight). 04-0.8% phosphorus dry weight content, the calcium content of less than 0.4% and the magnesium content 0.15% or more.1346 samples of Phalaenopsis tissue culture(TC) seedling collected from 3 orchid nurseries were detected by RT-PCR technique for Odontoglossum ringspot virus(ORSV)and Cymbidium mosaic virus(CymMV).Results showed that 92.8% samples were healthy seedlings
「植物種苗研究團隊-生技種苗檢測服務建置與產業推動生物技術課Industry extension and establishmen」所屬的資料集:「種苗研發成果」的其他資料
年度:
2013
計畫名稱_中文:
種子檢查技術研習
計畫名稱_英文:
Study on seed testing technology
計畫編號_中文:
102農科-4.2.1-種-X1
年度:
2011
計畫名稱_中文:
木瓜全兩性株基因體定序研究(委辦)
計畫名稱_英文:
Study on the whole genome sequence of all hermaphorodite papaya cultivar
計畫編號_中文:
100農科-1.1.8-種-X4
年度:
2013
計畫名稱_中文:
建立飼料玉米種子籌供體系
計畫名稱_英文:
Establishment of hybrid corn production and supplying system
計畫編號_中文:
102農科-9.2.1-種-X1
年度:
2010
計畫名稱_中文:
台灣植物資源產業化應用
計畫名稱_英文:
Application of industry of Taiwan vegetation resources
計畫編號_中文:
99農科-4.2.2-種-X6
年度:
2014
計畫名稱_中文:
建立硬質玉米種子籌供體系
計畫名稱_英文:
Establishment of hybrid corn production and supplying system
計畫編號_中文:
103農科-9.2.2-種-X1
年度:
2014
計畫名稱_中文:
番木瓜有機栽培及有機雜交種子生產研究
計畫名稱_英文:
Study on organic cultivation and organic papaya seed
計畫編號_中文:
103農科-9.2.7-種-X2
年度:
2013
計畫名稱_中文:
有機團隊-番木瓜有機栽培及有機雜交種子生產研究
計畫名稱_英文:
Study on organic cultivation and organic papaya seed production
計畫編號_中文:
102農科-9.2.4-種-X2
年度:
2011
計畫名稱_中文:
有機團隊-有機種衣劑開發及種子苗管理機制之建立
計畫名稱_英文:
Establishment of organic coating material and seedling management
計畫編號_中文:
100農科-4.2.5-種-X1
年度:
2013
計畫名稱_中文:
植物種苗研究團隊-亞太地區植物種苗產業研究與應用
計畫名稱_英文:
Plant nursery research group-Studying and application on plant seed industry in Asia-Pacicic region
計畫編號_中文:
102農科-9.2.3-種-X6
年度:
2010
計畫名稱_中文:
優質番木瓜品種選育、採種及栽培技術改進
計畫名稱_英文:
Good quality varieties selection and improvement the technology of cultivation and seed production o
計畫編號_中文:
99農科-4.2.2-種-X1
年度:
2010
計畫名稱_中文:
植物種苗團隊-基因轉殖作物種苗檢測監測體系之建立
計畫名稱_英文:
Establishment of detection and monitors system for genetically modified seed
計畫編號_中文:
99農科-1.1.6-種-X1
年度:
2011
計畫名稱_中文:
植物種苗團隊-生物技術於植物品種開發及種苗驗證之應用研究-植物種苗認驗證體系建構
計畫名稱_英文:
Construct the Accreditation system for plant seedlings
計畫編號_中文:
100農科-1.1.12-種-X1
年度:
2012
計畫名稱_中文:
作物遺傳種原收集、保存與利用
計畫名稱_英文:
Preservation, collection and application of germplasm
計畫編號_中文:
101農科-9.2.3-種-X7
年度:
2012
計畫名稱_中文:
雜糧作物種子調製倉儲技術改進研究
計畫名稱_英文:
Improvement of Seed process and storage techniques
計畫編號_中文:
101農科-9.2.1-種-X2
年度:
2013
計畫名稱_中文:
建構亞太植物種苗產業服務資訊平台
計畫名稱_英文:
Construction for Asia Pacific Seedlings Industry Services Platform
計畫編號_中文:
102農科-7.1.1-種-X1
年度:
2011
計畫名稱_中文:
作物種苗教育訓練效益評估之研究
計畫名稱_英文:
Benefit appraisal of crops seedling technical training
計畫編號_中文:
100農科-5.2.1-種-X1
年度:
2011
計畫名稱_中文:
植物種苗團隊-以知識密集服務為導向之種苗產業服務網站建置研究
計畫名稱_英文:
Establishment of the Knowledge-Intensive Service Website for Seedling Industry
計畫編號_中文:
100農科-6.1.1-種-X1
年度:
2011
計畫名稱_中文:
植物種苗團隊-植物品種檢定流程分析、人員訓練及分子標誌開發
計畫名稱_英文:
plant seed/seedling team – Analyzing the examining process flow, training faculty members and develo
計畫編號_中文:
100農科-1.1.9-種-X1
年度:
2014
計畫名稱_中文:
水稻種子影像辨識輔助系統之研究與開發
計畫名稱_英文:
A study and development of inspection system for rice seeds with image processing
計畫編號_中文:
103農科-7.1.1-種-X1
年度:
2010
計畫名稱_中文:
植物種苗團隊-利用分子標誌輔助育種技術平台
計畫名稱_英文:
The establishment of technique platform by marker-assisted selection
計畫編號_中文:
99農科-1.1.10-種-X1
年度:
2010
計畫名稱_中文:
植物種苗團隊-符合外銷國之種子苗驗證技術研發、制度研究與建置
計畫名稱_英文:
Establishment and Research of Seed and
Seedling Certification Techniques and Regulations
計畫編號_中文:
99農科-9.2.1-種-X2
年度:
2010
計畫名稱_中文:
草莓健康種苗產程管理技術開發(98-99產學合作)
計畫名稱_英文:
Healthy strawberry seedling production
and management for year -round
計畫編號_中文:
99農科-5.4.2-種-X2
年度:
2013
計畫名稱_中文:
花卉研究團隊-春石斛開花株生理指標及花期調節管理體系之建立
計畫名稱_英文:
Set up nobile-type dendrobium mature index and flowering regulation systerm
計畫編號_中文:
102農科-9.2.2-種-X4
年度:
2014
計畫名稱_中文:
基因轉殖及非基因轉殖種苗驗證及共存體系之建構-基因轉殖作物認驗證與檢監測模式之建立
計畫名稱_英文:
Construction of the certification and coexistence system for GM and non-GM seedlings
計畫編號_中文:
103農科-1.1.1-種-X1
年度:
2012
計畫名稱_中文:
植物種苗團隊-植物品種檢定流程分析、人員訓練及分子標誌開發
計畫名稱_英文:
plant seed/seedling team – Analyzing the examining process flow, training faculty members and develo
計畫編號_中文:
101農科-9.2.3-種-X1
年度:
2011
計畫名稱_中文:
植物種苗團隊-植物育種研發成果移轉機制建構及媒合平台建置
計畫名稱_英文:
Study on mechanism and platform for matching and transfer of COA's plant breeding achievements
計畫編號_中文:
100農科-4.2.2-種-X5
年度:
2010
計畫名稱_中文:
優質植物種苗量產體系建立
計畫名稱_英文:
Establishment of production system of
high quality seedlings
計畫編號_中文:
99農科-4.2.2-種-X5
年度:
2014
計畫名稱_中文:
蔬菜有機種衣劑及種子有機處理基準之研究
計畫名稱_英文:
Establishment of vegetables organic coating material and organic processing benchmark in seedling
計畫編號_中文:
103農科-9.2.7-種-X1
年度:
2014
計畫名稱_中文:
農業副產物應用於仙履蘭、春石斛、國蘭、文心蘭等蘭科作物種苗栽培介質之研發
計畫名稱_英文:
Use of agricultural byproducts as growing substrates for orchids seedling
計畫編號_中文:
103農科-9.2.12-種-X1
年度:
2014
計畫名稱_中文:
種子檢查技術研習
計畫名稱_英文:
Study on seed testing technology
計畫編號_中文:
103農科-4.2.1-種-X1
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