"Pollution source identification by microbial community profile (2/2)"
「"Pollution source identification by microbial community profile (2/2)"」於資料集「EPQSummary_EnvironmentalMonitoringAndTesting」由單位「行政院環境保護署」的陳先生所提供，聯繫電話是02-2311-7722#2386，最近更新時間為：2023-07-30 01:01:45。 欄位Project Title的內容是"Pollution source identification by microbial community profile (2/2)" ， 欄位Project Subject的內容是"This study investigates the possibility of applying microbial community profile changes to pollution source identification. In the first-year study, sediment microcosm was established and exposed to copper sulfate. In addition, water and sediments were sampled from a local river to verify the results. The results showed that Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, and Xanthobacteraceae can be found from microcosm, river sediments and factory discharge. And, the correlation analysis showed that Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, and Flavobacteriaceae were closely related to copper concentration. In the second year, sediments and water samples from both upstream and downstream of an actual industrial park discharging point were collected and analyzed. These samples will be used to verify the possibility of using the microbial change analysis as a pollution source identification. 16S-rRNA gene amplicon sequencing was performed for all 5 sediment samples collected from the river receiving industrial discharge and metagenomics function from each sample was estimated by PICRUSt (v1.1.3). In addition, whole sediment genome was also analyzed by illumnia shot gun sequencing. The amplicon sequencing results showed that Microscillaceae and Geobacteraceae were increased after discharging point. The Hydrogenophilaceae and Gallionaellaceae were two groups mostly affected by the industrial discharge. Especially, the Hydrogenophilaceae group is in agreement as the finding from the first year’s results. The results from PICRUSt estimation, there were 20 gene functions were affected by the industrial discharge, within those function, calcium signal pathway and photosynthesis – antenna proteins were the two major functions affected significantly by the industrial discharge, and may be considered as functional gene indicators in the future. From the whole genome analysis, the metal related (Cu, Zn, Cr) ATPase activity (transporting or exporting) gene expressed highest impact from the industrial discharge. It can also be considered and indicating functional gene for the future applications. " ， 欄位Project Year的內容是2018 ， 欄位Organizer的內容是"Environmental Analysis Laboratory" ， 欄位Executive Unit的內容是"國立臺灣大學" ， 欄位Reporting download URL的內容是https://epq.epa.gov.tw/project/filedownload.aspx?fid=185147 ， 欄位Publish Date的內容是20181229 。
Project Title
"Pollution source identification by microbial community profile (2/2)"
Project Subject
"This study investigates the possibility of applying microbial community profile changes to pollution source identification. In the first-year study, sediment microcosm was established and exposed to copper sulfate. In addition, water and sediments were sampled from a local river to verify the results. The results showed that Aeromonadaceae, Burkholderiales_incertae_sedis, Caldilineaceae, Chloroflexi, Firmicutes, Planctomycetaceae, Pseudomonodaceae, and Xanthobacteraceae can be found from microcosm, river sediments and factory discharge. And, the correlation analysis showed that Burkholderiaceae, Hydrogenophilaceae, Methylophilaceae, Chitinophagaceae, and Flavobacteriaceae were closely related to copper concentration. In the second year, sediments and water samples from both upstream and downstream of an actual industrial park discharging point were collected and analyzed. These samples will be used to verify the possibility of using the microbial change analysis as a pollution source identification. 16S-rRNA gene amplicon sequencing was performed for all 5 sediment samples collected from the river receiving industrial discharge and metagenomics function from each sample was estimated by PICRUSt (v1.1.3). In addition, whole sediment genome was also analyzed by illumnia shot gun sequencing. The amplicon sequencing results showed that Microscillaceae and Geobacteraceae were increased after discharging point. The Hydrogenophilaceae and Gallionaellaceae were two groups mostly affected by the industrial discharge. Especially, the Hydrogenophilaceae group is in agreement as the finding from the first year’s results. The results from PICRUSt estimation, there were 20 gene functions were affected by the industrial discharge, within those function, calcium signal pathway and photosynthesis – antenna proteins were the two major functions affected significantly by the industrial discharge, and may be considered as functional gene indicators in the future. From the whole genome analysis, the metal related (Cu, Zn, Cr) ATPase activity (transporting or exporting) gene expressed highest impact from the industrial discharge. It can also be considered and indicating functional gene for the future applications. "
Project Year
2018
Organizer
"Environmental Analysis Laboratory"
Executive Unit
"國立臺灣大學"
Reporting download URL
Publish Date
20181229
「"Pollution source identification by microbial community profile (2/2)"」所屬的資料集：「EPQSummary_EnvironmentalMonitoringAndTesting」的其他資料
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