"Quantum Dots-BioChip Detection System for Environmental Microbe
「"Quantum Dots-BioChip Detection System for Environmental Microbe」於資料集「EPQSummary_EnvironmentalMonitoringAndTesting」由單位「行政院環境保護署」的陳先生所提供，聯繫電話是02-2311-7722#2386，最近更新時間為：2023-07-30 01:01:45。 欄位Project Title的內容是"Quantum Dots-BioChip Detection System for Environmental Microbes (4/4)-The Flow Assay System of cytokines by Quantum Dots Detection SyStem" ， 欄位Project Subject的內容是"The main purpose of this project “Quantum Dots-BioChip Detection System for Environmental Microbes (4/4)- The Flow Assay System of cytokines by Quantum Dots Detection SyStem” is to combine two technologies included yeast screening and cytokine detection system. The double expression plasmid in yeast system can be applied for primary screening for environmental hormone and the cytokine detection system for the specific cytokine. The cytokine detection system will be applied by previous technology that was developed by previous EPA projects. This novel technology utilized microbead linked with specific oligonucleotide and detected by qutatum dots. The advantage of this is high accuracy for quantity and quality to monitor target samples. In double expression plasmid yeast system, we have finished the expression plasmid. This plasmid will express human estrogen receptor (hER-), estrogen responsive elements (EREs) and reporter gene including BGFP and β-galactosidase. For BGFP detection, those environmental hormones can be detected in our yeast expression system for linear detection between 10-2~10-9 g/L, and R2=0.88~0.98. For β-galactosidase detectin, those environmental hormone can be detected in our yeast expression system for linear detection between 10-2~10-7 g/L, and R2=0.81~0.99. To evaluate 5 cytokines incluing L1-, IL-6, LT-, TNF- and SPP1 in our microbead-QD system, the control RNAs were prepared by in vitro transcription assay. The detection limit of 5 cytokines is around 6.25 pg with R2=0.99。The specificity of those 5 cytokines is up to 96%. In addition, the A549 cells were treated with various drugs to induce the cytokines and tested in microbead-QD system, the data demonstrated our system has high sensitivity up to 10-10g with R2=0.92~0.99 without false negative. The comparison between Q-PCR and microbead-QD system showed the high relationship with R=0.71~0.99 except SPP1." ， 欄位Project Year的內容是2016 ， 欄位Organizer的內容是"Environmental Analysis Laboratory" ， 欄位Executive Unit的內容是"中原大學" ， 欄位Reporting download URL的內容是https://epq.epa.gov.tw/project/filedownload.aspx?fid=131366 ， 欄位Publish Date的內容是20170201 。
Project Title
"Quantum Dots-BioChip Detection System for Environmental Microbes (4/4)-The Flow Assay System of cytokines by Quantum Dots Detection SyStem"
Project Subject
"The main purpose of this project “Quantum Dots-BioChip Detection System for Environmental Microbes (4/4)- The Flow Assay System of cytokines by Quantum Dots Detection SyStem” is to combine two technologies included yeast screening and cytokine detection system. The double expression plasmid in yeast system can be applied for primary screening for environmental hormone and the cytokine detection system for the specific cytokine. The cytokine detection system will be applied by previous technology that was developed by previous EPA projects. This novel technology utilized microbead linked with specific oligonucleotide and detected by qutatum dots. The advantage of this is high accuracy for quantity and quality to monitor target samples. In double expression plasmid yeast system, we have finished the expression plasmid. This plasmid will express human estrogen receptor (hER-), estrogen responsive elements (EREs) and reporter gene including BGFP and β-galactosidase. For BGFP detection, those environmental hormones can be detected in our yeast expression system for linear detection between 10-2~10-9 g/L, and R2=0.88~0.98. For β-galactosidase detectin, those environmental hormone can be detected in our yeast expression system for linear detection between 10-2~10-7 g/L, and R2=0.81~0.99. To evaluate 5 cytokines incluing L1-, IL-6, LT-, TNF- and SPP1 in our microbead-QD system, the control RNAs were prepared by in vitro transcription assay. The detection limit of 5 cytokines is around 6.25 pg with R2=0.99。The specificity of those 5 cytokines is up to 96%. In addition, the A549 cells were treated with various drugs to induce the cytokines and tested in microbead-QD system, the data demonstrated our system has high sensitivity up to 10-10g with R2=0.92~0.99 without false negative. The comparison between Q-PCR and microbead-QD system showed the high relationship with R=0.71~0.99 except SPP1."
Project Year
2016
Organizer
"Environmental Analysis Laboratory"
Executive Unit
"中原大學"
Reporting download URL
Publish Date
20170201
「"Quantum Dots-BioChip Detection System for Environmental Microbe」所屬的資料集：「EPQSummary_EnvironmentalMonitoringAndTesting」的其他資料
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