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花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育品種改良保護課Breeding of Pachira macrocarpa via

「花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育品種改良保護課Breeding of Pachira macrocarpa via」於資料集「種苗研發成果」由單位「農業部」的陳乃華小姐所提供，聯繫電話是04-2582-5425，最近更新時間為：2023-07-13 01:04:11。欄位年度的內容是2012 ，欄位計畫名稱_中文的內容是花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育 ，欄位計畫名稱_英文的內容是Breeding of Pachira macrocarpa via chemical mutagenesis ，欄位計畫編號_中文的內容是101農科-9.2.2-種-X4 ，欄位計畫編號_英文的內容是101AS -9.2.2-SS-X4 ，欄位主辦單位_中文的內容是品種改良保護課 ，欄位主辦單位_英文的內容是Plant Breeding Section ，欄位主辦人_中文的內容是郭孄婷 ，欄位主辦人_英文的內容是Lan-Ting Kuo ，欄位中文摘要的內容是將採收之馬拉巴栗果實剖開，取出種子，以NaN3不同濃度進行浸種，分別為0 mM、0.5mM、1mM及2mM，浸種24hr、48hr，計算其發芽率並觀察誘變之結果，0 mM、0.5mM、1mM及2mM NaN3處理24hr，種子之發芽率分別為11.43%、10%、22.86%及11.76%，處理48hr後，種子之發芽率分別為0%、0%、1.52%、12.12%。目前已有三株變異株，變異之性狀分別為葉脈中間具有淺色斑紋及節間短縮，葉形細長捲曲。組織培養建立方面，未成熟種子切段進行癒合組織誘導，可觀察到各段節於傷口處有癒合組織產生，以含2,4-D 1mg/L之培養基效果較佳，2,4-D 2mg/L之處理，其培殖體易褐化，部分處理則有子葉轉綠之情形，逆分化效果較輕微。所產生之癒合組織多發生於切口處，呈現鬆散狀，無觀察到擬胚的產生，需進一步調整培養基，若以含2,4-D及2ip之培養基繼代培養，癒合組織可持續生長。，欄位英文摘要的內容是The seeds of Pachira macrocarpa were collected, and treated with 0mM,0.5mM, 1mM and 2mM for 24hrs or 48hrs. After treatments, the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 0, 0.5, 1, 2mM NaN3 for 24hrs were 1.43%, 10%,22.86% and 11.76%. After 48hr, the germination rate were 0%, 0%, 1.52%,12.12%. There have been three mutated plants induced by NaN3 treatment.One is with leaf variegation along the central vines and the others are with thinner and undulated leaves. As to callus induction, medium with 12,4-D 1mg/L had the best effect on callus induction, and 2,4-D 1mg/L caused browning frequently. Under the other treatments, explants grew greenly and had less calli. Most of calli formed from the cut places of explants, and were friable without embro-like body. Calli subcultured in medium with 2,4-D and 2ip can keep growing, but still without embryo-like body. 。

年度

2012

計畫名稱_中文

花卉團隊-以化學性誘變法進行馬拉巴栗新品系選育

計畫名稱_英文

Breeding of Pachira macrocarpa via chemical mutagenesis

計畫編號_中文

101農科-9.2.2-種-X4

計畫編號_英文

101AS -9.2.2-SS-X4

主辦單位_中文

品種改良保護課

主辦單位_英文

Plant Breeding Section

主辦人_中文

郭孄婷

主辦人_英文

Lan-Ting Kuo

中文摘要

將採收之馬拉巴栗果實剖開，取出種子，以NaN3不同濃度進行浸種，分別為0 mM、0.5mM、1mM及2mM，浸種24hr、48hr，計算其發芽率並觀察誘變之結果，0 mM、0.5mM、1mM及2mM NaN3處理24hr，種子之發芽率分別為11.43%、10%、22.86%及11.76%，處理48hr後，種子之發芽率分別為0%、0%、1.52%、12.12%。目前已有三株變異株，變異之性狀分別為葉脈中間具有淺色斑紋及節間短縮，葉形細長捲曲。組織培養建立方面，未成熟種子切段進行癒合組織誘導，可觀察到各段節於傷口處有癒合組織產生，以含2,4-D 1mg/L之培養基效果較佳，2,4-D 2mg/L之處理，其培殖體易褐化，部分處理則有子葉轉綠之情形，逆分化效果較輕微。所產生之癒合組織多發生於切口處，呈現鬆散狀，無觀察到擬胚的產生，需進一步調整培養基，若以含2,4-D及2ip之培養基繼代培養，癒合組織可持續生長。

英文摘要

The seeds of Pachira macrocarpa were collected, and treated with 0mM,0.5mM, 1mM and 2mM for 24hrs or 48hrs. After treatments, the hygrochastic seeds were planted apart. Then counted the germination rates of seeds and observed if there was mutation. The germination rate of hygrachastic seeds treated with 0, 0.5, 1, 2mM NaN3 for 24hrs were 1.43%, 10%,22.86% and 11.76%. After 48hr, the germination rate were 0%, 0%, 1.52%,12.12%. There have been three mutated plants induced by NaN3 treatment.One is with leaf variegation along the central vines and the others are with thinner and undulated leaves. As to callus induction, medium with 12,4-D 1mg/L had the best effect on callus induction, and 2,4-D 1mg/L caused browning frequently. Under the other treatments, explants grew greenly and had less calli. Most of calli formed from the cut places of explants, and were friable without embro-like body. Calli subcultured in medium with 2,4-D and 2ip can keep growing, but still without embryo-like body.