植物種苗團隊-作物種苗微體繁殖技術之開發與改進繁殖技術課The study of healthy seedling produc
「植物種苗團隊-作物種苗微體繁殖技術之開發與改進繁殖技術課The study of healthy seedling produc」於資料集「種苗研發成果」由單位「農業部」的陳乃華 小姐所提供,聯繫電話是04-2582-5425,最近更新時間為:2023-07-13 01:04:11。 欄位年度的內容是2012 , 欄位計畫名稱_中文的內容是植物種苗團隊-作物種苗微體繁殖技術之開發與改進 , 欄位計畫名稱_英文的內容是The study of healthy seedling production by tissue culture , 欄位計畫編號_中文的內容是101農科-9.1.1-種-X2 , 欄位計畫編號_英文的內容是101AS -9.1.1-SS-X2 , 欄位主辦單位_中文的內容是繁殖技術課 , 欄位主辦單位_英文的內容是Propagation Technology Section , 欄位主辦人_中文的內容是陳哲仁 , 欄位主辦人_英文的內容是Jen-Ren Chen , 欄位中文摘要的內容是本試驗建立春石斛9117品種組織培養繁殖技術及植株苗期肥培管理條件。使用添加細胞分裂素BA或TDZ之培養基,可顯著的促進芽體增殖數,以B4、T2培養2個月每培殖體平均可形成3.12芽、3.23芽,顯著高於對照組之1.6芽。增殖之芽體經移至添加NAA和香蕉粉之培養基培養,具有誘導根系形成之效果,以N1B3培養基培養2個月後根數達5.6根,根長達4.1 cm且發根率達100.0%為最佳。將組培苗出瓶種植10個月之植株,進行不同氮、磷、鉀比例之營養液處理,試驗對其苗期生長、開花表現之影響,結果顯示,苗期植株外表性狀之生長以低量的磷、鉀肥處理(N1-P1-K1、N1-P2-K1)較佳,然後續之開花表現則以苗期施用中量之磷、鉀肥(N1-P2-K2)之表現最佳。訪視春石斛蘭業者,確認主要病害為炭疽病及葉斑病。採樣病株,進行組織分離,得到4株炭疽病菌,並回接5品種石斛蘭葉片,確認病原性。
本研究為探討3品種鳳梨不定芽形成,以0.5 mg/L TDZ 處理之側芽培植體癒傷組織誘導率最高,為80%,培養3 週後已有不定芽形成。而不定芽形成率則以添加3.0mg/L BA最高,為98%。在不定芽誘導發根試驗結果顯示以2.0 mg/L NAA或IAA之發根率95% 為最佳,平均發根數有2.1根形成。
種苗金皇一號石斛冷水萃取物可以活化小鼠巨噬細胞(RAW264.7 cells)促進NO的釋放,而且也可以明顯促進TNF-α 和IL-6的釋放。
, 欄位英文摘要的內容是An efficient in vitro propagation protocol for nobile-type dendrobium was established. In this study, a shoot proliferation medium supplemented with different concentration of cytokinins (BA or TDZ) was evaluated its effect on shoot proliferation. The results indicated that using shoot proliferation medium B4 or T2 were optimal for shoot proliferation with shoot proliferation number per explant of 3.12, 3.23. The rooting rate of plantlets were 100% after two months rooting in the rooting medium –N1B3, the average root number and root length of plantlets were 5.6 and 4.1 cm, respectively. In addition, the purpose of this study was to evaluate the effect of fertilizers with different rate of nitrogen (N), phosphorus (P), and potassium (K) on the growth and flowering of the nobile-type dendrobium. The results show that treatments of N1-P1-K1 and N1-P2-K1 were optimal for growth, but the N1-P2-K2 treatment was optimal for flowering.
We have visited 2 Dendrobium entertainments, confirming the main
diseases are anthracnose and Pseudocercospra leaf spots. By sampling diseased plants and tissue separating, 4 strains of Colletotrichum spp.were obtained. Leaves of 5 Dendrobium varieties were inoculated with these strains for making sure their pathogenicity.The objectives of the present study were to develop culture method for shoot formation in 3cultivars of pineapple. Treatment of 0.5 mg/L TDZ was found best in callus induction from lateral bud explants, induction percentage reached 80% , shoots were formed after culture for 3 weeks.The highest percent shoot formation which is 98% was found in the treatment of 3.0 mg/L BA. In addition, treatment of 2.0 mg/L NAA or IAAwas found best in root formation from shoot, formation percentage reached 95% , the average roots formed per shoot were 2.1。
A water-soluble crude extracts was obtained from Dendrobium Taiseed Tosnobile by cold water extraction(CWDTT). The data was showed the activities to stimulate macrophages and enhanced the release of NO from RAW264.7 macrophages. Furthermore, the concentration of Interleukin-6(IL-6) and TNF-α were significantly stimulated after exposed to CWDTT
。
年度
2012
計畫名稱_中文
植物種苗團隊-作物種苗微體繁殖技術之開發與改進
計畫名稱_英文
The study of healthy seedling production by tissue culture
計畫編號_中文
101農科-9.1.1-種-X2
計畫編號_英文
101AS -9.1.1-SS-X2
主辦單位_中文
繁殖技術課
主辦單位_英文
Propagation Technology Section
主辦人_中文
陳哲仁
主辦人_英文
Jen-Ren Chen
中文摘要
本試驗建立春石斛9117品種組織培養繁殖技術及植株苗期肥培管理條件。使用添加細胞分裂素BA或TDZ之培養基,可顯著的促進芽體增殖數,以B4、T2培養2個月每培殖體平均可形成3.12芽、3.23芽,顯著高於對照組之1.6芽。增殖之芽體經移至添加NAA和香蕉粉之培養基培養,具有誘導根系形成之效果,以N1B3培養基培養2個月後根數達5.6根,根長達4.1 cm且發根率達100.0%為最佳。將組培苗出瓶種植10個月之植株,進行不同氮、磷、鉀比例之營養液處理,試驗對其苗期生長、開花表現之影響,結果顯示,苗期植株外表性狀之生長以低量的磷、鉀肥處理(N1-P1-K1、N1-P2-K1)較佳,然後續之開花表現則以苗期施用中量之磷、鉀肥(N1-P2-K2)之表現最佳。訪視春石斛蘭業者,確認主要病害為炭疽病及葉斑病。採樣病株,進行組織分離,得到4株炭疽病菌,並回接5品種石斛蘭葉片,確認病原性。 本研究為探討3品種鳳梨不定芽形成,以0.5 mg/L TDZ 處理之側芽培植體癒傷組織誘導率最高,為80%,培養3 週後已有不定芽形成。而不定芽形成率則以添加3.0mg/L BA最高,為98%。在不定芽誘導發根試驗結果顯示以2.0 mg/L NAA或IAA之發根率95% 為最佳,平均發根數有2.1根形成。 種苗金皇一號石斛冷水萃取物可以活化小鼠巨噬細胞(RAW264.7 cells)促進NO的釋放,而且也可以明顯促進TNF-α 和IL-6的釋放。
英文摘要
An efficient in vitro propagation protocol for nobile-type dendrobium was established. In this study, a shoot proliferation medium supplemented with different concentration of cytokinins (BA or TDZ) was evaluated its effect on shoot proliferation. The results indicated that using shoot proliferation medium B4 or T2 were optimal for shoot proliferation with shoot proliferation number per explant of 3.12, 3.23. The rooting rate of plantlets were 100% after two months rooting in the rooting medium –N1B3, the average root number and root length of plantlets were 5.6 and 4.1 cm, respectively. In addition, the purpose of this study was to evaluate the effect of fertilizers with different rate of nitrogen (N), phosphorus (P), and potassium (K) on the growth and flowering of the nobile-type dendrobium. The results show that treatments of N1-P1-K1 and N1-P2-K1 were optimal for growth, but the N1-P2-K2 treatment was optimal for flowering. We have visited 2 Dendrobium entertainments, confirming the main diseases are anthracnose and Pseudocercospra leaf spots. By sampling diseased plants and tissue separating, 4 strains of Colletotrichum spp.were obtained. Leaves of 5 Dendrobium varieties were inoculated with these strains for making sure their pathogenicity.The objectives of the present study were to develop culture method for shoot formation in 3cultivars of pineapple. Treatment of 0.5 mg/L TDZ was found best in callus induction from lateral bud explants, induction percentage reached 80% , shoots were formed after culture for 3 weeks.The highest percent shoot formation which is 98% was found in the treatment of 3.0 mg/L BA. In addition, treatment of 2.0 mg/L NAA or IAAwas found best in root formation from shoot, formation percentage reached 95% , the average roots formed per shoot were 2.1。 A water-soluble crude extracts was obtained from Dendrobium Taiseed Tosnobile by cold water extraction(CWDTT). The data was showed the activities to stimulate macrophages and enhanced the release of NO from RAW264.7 macrophages. Furthermore, the concentration of Interleukin-6(IL-6) and TNF-α were significantly stimulated after exposed to CWDTT
「植物種苗團隊-作物種苗微體繁殖技術之開發與改進繁殖技術課The study of healthy seedling produc」所屬的資料集:「種苗研發成果」的其他資料
年度:
2011
計畫名稱_中文:
種子調製倉儲技術
計畫名稱_英文:
Seed process and storage improvement
techniques
計畫編號_中文:
100農科-4.2.3-種-X5
年度:
2011
計畫名稱_中文:
仙履蘭及熱帶球根花卉品種改良與種苗生產技術開發
計畫名稱_英文:
Improvement on slipper orchid and tropic bulb flowers breeding and establishment the seedling produc
計畫編號_中文:
100農科-4.2.2-種-X3
年度:
2012
計畫名稱_中文:
有機農業團隊-蔬菜種子有機種衣劑與種子有機處理基準之研究
計畫名稱_英文:
Establishment of vegetables organic coating material and organic processing benchmark in seedling
計畫編號_中文:
101農科-9.2.4-種-X1
年度:
2010
計畫名稱_中文:
植物種苗團隊-植物新品種檢定技術之開發與執行
計畫名稱_英文:
The Technical Development and DUS Test Execution of Plant Variety Protection System
計畫編號_中文:
99農科-4.2.2-種-X7
年度:
2012
計畫名稱_中文:
台灣香藥草植物資源開發利用
計畫名稱_英文:
Development and utilization of indigenous aromatic herbs resources in Taiwan
計畫編號_中文:
101農科-9.2.3-種-X8
年度:
2010
計畫名稱_中文:
作物種苗知識傳播之執行效益評估
計畫名稱_英文:
Benefit appraisal of crops seedling knowledge dissemination
計畫編號_中文:
99農科-5.2.1-種-X1
年度:
2012
計畫名稱_中文:
組織培養節能設備及技術之開發
計畫名稱_英文:
Energy-saving equipment and technology development for tissue culture
計畫編號_中文:
101農科-14.1.5-種-X1
年度:
2011
計畫名稱_中文:
木瓜全兩性株基因體定序研究(委辦)
計畫名稱_英文:
Study on the whole genome sequence of all hermaphorodite papaya cultivar
計畫編號_中文:
100農科-1.1.8-種-X4
年度:
2013
計畫名稱_中文:
有機團隊-番木瓜有機栽培及有機雜交種子生產研究
計畫名稱_英文:
Study on organic cultivation and organic papaya seed production
計畫編號_中文:
102農科-9.2.4-種-X2
年度:
2012
計畫名稱_中文:
植物種苗團隊-蔬菜品種改良及採種技術研究
計畫名稱_英文:
Study on improvement of vegetable varities and technology of seed production
計畫編號_中文:
101農科-9.2.2-種-X2
年度:
2013
計畫名稱_中文:
植物種苗研究團隊-果菜類作物品種改良及採種技術改進
計畫名稱_英文:
Study on improvement of fruit vegetable varieties and seed production technology
計畫編號_中文:
102農科-9.2.2-種-X2
年度:
2012
計畫名稱_中文:
植物種苗團隊-仙履蘭產業技術開發及應用
計畫名稱_英文:
Paphiopedilum industry technology
development and application
計畫編號_中文:
101農科-9.1.1-種-X3
年度:
2011
計畫名稱_中文:
植物種苗團隊-仙履蘭產業技術開發及應用
計畫名稱_英文:
Paphiopedilum industry technology
development and application
計畫編號_中文:
100農科-1.1.1-種-X3
年度:
2011
計畫名稱_中文:
植物種苗團隊-作物種苗微體繁殖技術之開發與改進
計畫名稱_英文:
The study of healthy seedling production by tissue culture
計畫編號_中文:
100農科-1.1.1-種-X2
年度:
2011
計畫名稱_中文:
植物種苗團隊-提升植物種苗產業經營管理能力輔導策略研究
計畫名稱_英文:
Enhance the ability of plant seed industry management counseling Strategy
計畫編號_中文:
100農科-5.2.1-種-X2
年度:
2013
計畫名稱_中文:
植物種苗研究團隊-生技種苗檢測服務建置與產業推動
計畫名稱_英文:
Industry extension and establishment of
biotech seedling detection serviceplant seed/seedling team
計畫編號_中文:
102農科-1.1.2-種-X1
年度:
2010
計畫名稱_中文:
優質植物種苗量產體系建立
計畫名稱_英文:
Establishment of production system of
high quality seedlings
計畫編號_中文:
99農科-4.2.2-種-X5
年度:
2010
計畫名稱_中文:
建立有機栽培之種子苗生產管理機制
計畫名稱_英文:
Study on the management mechanism for organic seeds and seedlings growing
計畫編號_中文:
99農科-4.2.2-種-X9
年度:
2012
計畫名稱_中文:
植物種苗團隊-番木瓜種苗七號全兩性株調控基因分析與產業應用
計畫名稱_英文:
Analysis of regulation genes of allhermaphrodite papaya cv. TSS No.7 and its application on industry
計畫編號_中文:
101農科-1.1.2-種-X2
年度:
2014
計畫名稱_中文:
農業副產物應用於仙履蘭、春石斛、國蘭、文心蘭等蘭科作物種苗栽培介質之研發
計畫名稱_英文:
Use of agricultural byproducts as growing substrates for orchids seedling
計畫編號_中文:
103農科-9.2.12-種-X1
年度:
2014
計畫名稱_中文:
提升種子檢查量能模式之建立
計畫名稱_英文:
Establishment of the testing system to promote seed test capacity
計畫編號_中文:
103農科-6.2.3-種-X5
年度:
2011
計畫名稱_中文:
植物種苗團隊-植物育種研發成果移轉機制建構及媒合平台建置
計畫名稱_英文:
Study on mechanism and platform for matching and transfer of COA's plant breeding achievements
計畫編號_中文:
100農科-4.2.2-種-X5
年度:
2013
計畫名稱_中文:
建構亞太植物種苗產業服務資訊平台
計畫名稱_英文:
Construction for Asia Pacific Seedlings Industry Services Platform
計畫編號_中文:
102農科-7.1.1-種-X1
年度:
2011
計畫名稱_中文:
植物種苗團隊-基因轉殖作物種苗檢測監測體系之建立
計畫名稱_英文:
The establishment of detection and monitors system for genetically modified seedling
計畫編號_中文:
100農科-1.1.6-種-X1
年度:
2012
計畫名稱_中文:
種子檢查技術研習
計畫名稱_英文:
Study on seed testing technology
計畫編號_中文:
101農科-4.2.1-種-X1
年度:
2013
計畫名稱_中文:
花卉研究團隊-春石斛開花株生理指標及花期調節管理體系之建立
計畫名稱_英文:
Set up nobile-type dendrobium mature index and flowering regulation systerm
計畫編號_中文:
102農科-9.2.2-種-X4
年度:
2010
計畫名稱_中文:
植物種苗團隊-赴荷蘭、法國研習植物品種保護技術
計畫名稱_英文:
The study of technique for plant variety protection by attending to Nertherlands and France
計畫編號_中文:
99農科-4.1.1-種-X1
年度:
2011
計畫名稱_中文:
綠肥作物開發與利用
計畫名稱_英文:
The exploitation of green manure crop
計畫編號_中文:
100農科-4.2.4-種-X1
年度:
2014
計畫名稱_中文:
耐濕冷馬鈴薯品種選育
計畫名稱_英文:
Wet and cold resistant breeding for potato
計畫編號_中文:
103農科-14.1.2-種-X1
年度:
2014
計畫名稱_中文:
赴荷蘭建置雙方蝴蝶蘭DNA資料庫
計畫名稱_英文:
Established the Phalaenopsis DNA database of both sides in Netherlands
計畫編號_中文:
103農科-4.2.1-種-X2
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